Determination of Amphetamine and Methamphetamine by Dispersive Liquid Phase Micro-extraction: A Novel Experimental Approach

Authors

  • Daruish Badakhshan Department of Chemistry, Faculty of Science, Islamic Azad University. Arak, Iran.
  • Majid Ramezani Department of Chemistry, Faculty of Science, Islamic Azad University. Arak, Iran.
Abstract:

Background: A syringe to syringe dispersive liquid phase micro-extraction-floating organic drop was applied and used for the simultaneous extraction and pre-concentration of trace amounts of amphetamine (AMP) and methamphetamine (MAMP) in urine samples. The extracted analytes were determined by high performance liquid chromatography along with diode array detection. Materials & Methods: In this study, n-hexane was selected as the extraction solvent without the need to use dispersive solvent. The analytical parameters affecting the micro-extraction efficiency, including pH of sample solution, extraction solvent volume, the cycles of extraction and time of centrifugation were investigated and optimized by screening and optimization experimental design methods. Results: Underoptimal conditions, the calibration curve had a linear range of 2 to 100 μg/L with the determination coefficient of R2 = 99.8 and R2 = 99.6 for AMP and MAMP, respectively. The limit of detection was 2μg/L for AMP and MAMP, and the enrichment factor was 75 and 68 for AMP and MAMP, respectively. Conclusions: This method is very simple, rapid and has been successfully used for pre-concentration and measurement of the analytes in urine samples, which is important to forensic studies.

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Journal title

volume 14  issue 4

pages  0- 0

publication date 2020-10

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